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Stem Cell Alternative 2i inhibitor Set
Set of 2 Inhibitors
CGP 77675 (Axon 2097)
CHIR99021 (Axon 1386)
(2 mg or 5 mg of each inhibitor)
* Two Inhibitors are in powder form (high purity: 99%)
Ready to be reconstituted freshly
to their 10 mM solutions in DMSO respectively.
Expert Recommendation!
Description
Stem Cell Alternative 2i inhibitor Set combines the SRC inhibitor CGP77675 (Axon 2097) with the GSK-3 inhibitor CHIR99021 (Axon 1386) as an alternative 2i strategy for modulating self-renewal and differentiation-associated signaling. It provides a pathway-diverse alternative to the conventional MEK/GSK-3 2i combination (Axon 2128).
The dual inhibition of Src and GSK-3 signaling by CGP77675 and CHIR99021 (termed alternative 2i) was found to maintain mouse embryonic stem cell (mESC) self-renewal and pluripotency marker expression as efficiently as the dual inhibition of MEK and GSK3 by PD0325901 and CHIR99021 (conventional 2i, Axon 2128). This alternative 2i method provides a versatile tool not only for the maintenance of mESCs in serum-free conditions but also for the derivation of ESCs from mouse embryos. It is relevant for comparing alternative pluripotency-supporting inhibitor combinations.
Key Features
- Alternative 2i stem cell inhibitor combination
- Combines SRC inhibition with GSK-3 inhibition
- Includes CGP77675 and CHIR99021
- Useful comparator to conventional MEK/GSK-3 2i conditions
Applications
- Stem cell maintenance protocol optimization
- Pluripotency signaling comparisons
- SRC and GSK-3 pathway studies
- Differentiation-control experiments in stem cell culture
More Information
| Parent CAS No. | N.A. |
|---|---|
| Chemical Name | N.A. |
| MFCD | N.A. |
| Short Description | Stem cell inhibitor set |
References
- T. Shimizu et al. Dual inhibition of Src and GSK3 maintains mouse embryonic stem cells, whose differentiation is mechanically regulated by Src signaling. Stem Cells 2012, 30, 1394-1404.
- List of publications using CHIR99021 (Axon 1386) purchased from Axon Medchem
- List of publications using CGP 77675 (Axon 2097) purchased from Axon Medchem
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