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Stem Cell LSC inhibitor Set
Set of 3 Inhibitors
LDN 193189 HCl (Axon 1509)
SB 431542 (Axon 1661)
CHIR99021 (Axon 1386)
(2 mg or 5 mg of each inhibitor)
* Three Inhibitors are in powder form (high purity: 99%)
Ready to be reconstituted freshly
to their 10 mM solutions in DMSO respectively.
Expert Recommendation!
Description
A convenient set of BMP inhibitor LDN 193189 hydrochloride (Axon 1509), TGF-beta inhibitor SB 431542 (Axon 1661) and GSK-3 inhibitor CHIR99021 (Axon 1386) (termed as LSC or LSB/CHIR by their name abbreviation), for differentiation of human pluripotent stem cells (hPSCs). Combining two-factor neuronal programming with small molecule–based inhibition of GSK-3β by CHIR99021 (2 µM) and SMAD signaling by LDN 193189 (0.5 µM) and SB 431542 (10 µM), converted postnatal human fibroblasts into functional neuron-like cells with yields up to >200% and neuronal purities up to >80%.
The LSC (or LSB/CHIR) inhibitor cocktail protocol can be further extended; with adding SU 5402 (Axon 1667) and DAPT (Axon 1484) to form LSB3i, a 5i inhibitor set (Axon 5007), for rapid differentiating hPSCs into nociceptors.
Read more: Additional Product Information
Show all Axon Ligand™ Sets
More Information
| Parent CAS No. | N.A. |
|---|---|
| Chemical Name | N.A. |
| MFCD | N.A. |
| Short Description | Stem cell inhibitor set |
References
- J. Ladewig et al. Small molecules enable highly efficient neuronal conversion of human fibroblasts. Nat Methods. 2012, 9, 575-578.
- S.M. Chambers et al. Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nature Biotechnol. 2012, 30, 715-720.
- List of publications using LDN 193189 hydrochloride (Axon 1509) purchased from Axon Medchem
- List of publications using CHIR99021 (Axon 1386) purchased from Axon Medchem
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