Potent and selective Src family kinase (SFK) inhibitor; CGP77675 inhibited phosphorylation of peptide substrates and autophosphorylation of purified Src (IC50: 5-20 and 40 nM, respectively). The dual inhibition of Src and GSK-3 signaling by CGP 77675 and CHIR 99021 (termed alternative 2i, Axon 2130) was found to maintain mouse embryonic stem cell (mESC) self-renewal and pluripotency marker expression as efficiently as the dual inhibition of MAPK and GSK-3 by PD 0325901 and CHIR99021 (conventional 2i, Axon 2128). This alternative 2i method provides a versatile tool not only for the maintenance of mESCs in serum-free conditions but also for the derivation of ESCs from mouse embryos.

*Promotion: CGP 77675 is also part of Inhibitor Set(s):

KEYWORDS: CGP 77675 | supplier | Src inhibitor | CGP77675 | CGP-77675 | CAS [234772-64-6] | Non Selective | SRC | Inhibitor | Enzymes