Potent and selective inhibitor of B-Raf kinase (Kd value 0.3 nM for BRAF), devoid of significant activity against a wide panel of enzymes, including p38α, GSK3β, and Lck. SB590885 maintains OCT4-ΔPE-GFP reporter activity and pluripotency gene expression in human ESCs after removal of exogenous KLF2 and NANOG expression, preserving the best colony morphology and proliferation. 

SB 590885 is frequently used in a combination of five compounds, including inhibitors of MEK, GSK-3β, BRAF, ROCK, and SRC, which support the expansion of viable OCT4-ΔPE-GFP+ human pluripotent cells after exogenous transcription factor expression has been removed:

(alternatively, CHIR99021 (Axon 1386) could be used as GSK-3β inhibitor)

Naive Stem Cell 5i inhibitor Set is commercially available (Axon 5011)

KEYWORDS: SB 590885 | Supplier | BRAF inhibitor | SB590885 | CAS [405554-55-4] | MEK | RAF | Inhibitor | pluripotent | ESC | stem cell | proliferation | MAPK | cancer | tumor