Positive-sense single stranded RNA (+RNA) viruses replicate at specialized compartments termed replication factories or membranous webs. These membrane-derived compartments are specifically enriched in the phosphatidylinositol 4-phosphate (PI4P) lipid. Humans have four kinases that synthesize PI4P, two typical lipid kinases, PI4KA and PI4KB (also known as PI4K IIIα and PI4K IIIβ), and two type II (or atypical) kinases, PI4K2A and PI4K2B (also known as PI4K IIα and PI4K IIβ). It is generally accepted that most +RNA viruses hijack PI4K kinase to produce PI4P-enriched membranes. Therefore, PI4Ks are considered a potential pharmacological target because of their importance in the life cycle of various +RNA viruses.[1]