The enzyme Hedgehog acyltransferase (HHAT; EC 2.3.1.) catalyzes the attachment of palmitate to the N-terminal cysteine of Sonic hedgehog (ShhN, a Shh precursor) via an amide bond. Mature Shh is a secreted signaling protein that is essential for proper embryonic development. In adults, aberrant Shh signaling drives initiation and maintenance of medulloblastoma and basal cell carcinoma and has been implicated in the progression of prostate cancer, gastrointestinal tumors and pancreatic cancer.
 E. Petrova et al. Inhibitors of Hedgehog acyltransferase block Sonic Hedgehog signaling. Nat. Chem. Biol. 2013, 9, 247-249.