Luna, M. C., Mars, M., Jakobs, C. E., Huffels, C. F., Ermakov, A., Hol, E. M., & Pasterkamp, R. J. (2025). Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures. STAR protocols, 6(3).

* SB431542 and Y27632 from Axon Medchem

 

Giacomoni, J., Laurin, K., Parmar, M., & Habekost, M. (2025). Protocol for 3D direct reprogramming of human glia into dopaminergic neurons with gene expression and immunocytochemistry validation. STAR protocols, 6(4).

* LSC: LDN193189, SB431542 and CHIR99021 from Axon Medchem

 

Rossignoli, G., Oberhuemer, M., Brun, I. S., Zorzan, I., Osnato, A., Wenzel, A., ... & Martello, G. (2025). Feeder-free culture of naive human pluripotent stem cells retaining embryonic, extraembryonic and blastoid generation potential. bioRxiv, 2025-01.

* PD0325901, XAV939, GO6983, Y27632, CHIR99021, A83-01, SB431542, PD173074 from Axon Medchem

 

Van Nerum, K., Wenzel, A., Argemi-Muntadas, L., Kafkia, E., Drews, A., Brun, I. S., ... & Żylicz, J. J. (2025). α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells. Nature Cell Biology, 27(5), 749-761.

* PD0325901, CHIR99021, GO6983, XAV939, SB431542, Y27632, A83-01, Valproic acid from Axon Medchem

 

Arboit, M., Zorzan, I., Pensabene, E., Pellegrini, M., Bertelli, F., Panebianco, G., ... & Martello, G. (2025). KLF7 is a general inducer of human pluripotency. EMBO reports, 26(22), 5372.

* PD0325901, XAV939, GO6983, Y27632, SB431542, CHIR99021, A83-01 from Axon Medchem

 

Spijkers, X. M., Pasteuning-Vuhman, S., Timmers, A., Vulto, P., Wevers, N. R., & Pasterkamp, R. J. (2025). A comprehensive overview of the generation of iPSC-derived motor neurons and their incorporation in neuromuscular junction and microfluidic models. Microfluidic models to study the nervous system and its connection to the periphery, 39.

* CHIR99021, Y27632, SB431542 from Axon Medchem

 

Motoike, S., Inada, Y., Toguchida, J., Kajiya, M., & Ikeya, M. (2025). Jawbone-like organoids generated from human pluripotent stem cells. Nature Biomedical Engineering, 1-19.

* SB431542 and CHIR99021 from Axon Medchem

 

Breunig, M., Hohwieler, M., Haderspeck, J., von Zweydorf, F., Hauff, N., Pasquini, L. P., ... & Kleger, A. (2025). PPDPF is not a key regulator of human pancreas development. PLoS Genetics, 21(4), e1011657.

* CHIR99021 and SB431542 from Axon Medchem

 

Kohler, K. T., Kim, J., Villadsen, R., Rønnov-Jessen, L., & Petersen, O. W. (2024). Oncogene activated human breast luminal progenitors contribute basally located myoepithelial cells. Breast Cancer Research, 26(1), 183.

* SB431542 and Y-27632 from Axon Medchem

 

Onfray, C., Chevolleau, S., Moinard, E., Girard, O., Mahadik, K., Allsop, R., ... & David, L. (2024). Unraveling hallmark suitability for staging pre-and post-implantation stem cell models. Cell Reports, 43(5).

*Y-27632, CHIR99021, PD0325901, SB431542, A83-01, GO6983, XAV939, IWR-endo-1 from Axon Medchem

 

Warin, J., Vedrenne, N., Tam, V., Zhu, M., Yin, D., Lin, X., ... & Camus, A. (2024). In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells. Iscience, 27(2).

* SB431542, CHIR99021 from Axon Medchem

 

Giacomoni, J., Bruzelius, A., Habekost, M., Kajtez, J., Ottosson, D. R., Fiorenzano, A., ... & Parmar, M. (2024). 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Cell reports methods, 4(9).

* LSC: LDN193189, SB431542 and CHIR99021 from Axon Medchem

 

Spathopoulou, A., Podlesnic, M., De Gaetano, L., Kirsch, E. M., Tisch, M., Finotello, F., ... & Edenhofer, F. (2024). Single-cell profiling of reprogrammed human neural stem cells unveils high similarity to neural progenitors in the developing central nervous system. Stem Cell Reviews and Reports, 20(5), 1325-1339.

*CHIR99021 and SB431542 from Axon Medchem

 

Jacob, R. S., Dema, A., Chérot, H., Dumesnil, C., Cohen, S., Shalom, H. S., ... & Selenko, P. (2024). α-Synuclein acts as a cholesteryl-ester sensor on lipid droplets regulating organelle size and abundance. bioRxiv, 2024-06.

* Y27632, LDN193189, SB431542, Purmorphamine and DAPT from axon Medchem

 

Vieira de Sá, R., Sudria-Lopez, E., Cañizares Luna, M., Harschnitz, O., van den Heuvel, D. M., Kling, S., ... & Pasterkamp, R. J. (2024). ATAXIN-2 intermediate-length polyglutamine expansions elicit ALS-associated metabolic and immune phenotypes. Nature communications, 15(1), 7484.

* SB431542 and Y27632 from Axon Medchem

 

Laterza, C., Cesare, E., Stuart, H. T., D’Ercole, M., Gesualdo, A., La Barbera, M. G., ... & Elvassore, N. (2024). From naïve pluripotency to human neural organoids through a three-dimensional morphogenetic continuum. bioRxiv, 2024-11.

* SB431542 and XAV939 from Axon Medchem

 

Sonsalla, G., Malpartida, A. B., Riedemann, T., Gusic, M., Rusha, E., Bulli, G., ... & Masserdotti, G. (2024). Direct neuronal reprogramming of NDUFS4 patient cells identifies the unfolded protein response as a novel general reprogramming hurdle. Neuron, 112(7), 1117-1132.

*LSC: LDN193189, SB431542 and CHIR99021 from Axon Medchem

 

Cesana, M., Tufano, G., Panariello, F., Zampelli, N., Soldati, C., Mutarelli, M., ... & Ballabio, A. (2024). TFEB controls syncytiotrophoblast formation and hormone production in placenta. Cell Death & Differentiation, 31(11), 1439-1451.

× SB431542, CHIR99021, PD0325901, A83-01, Y-27632 from Axon Medchem

 

Arboit, M., Zorzan, I., Pellegrini, M., Martini, P., Carbognin, E., & Martello, G. (2023). KLF7 is a general inducer of human pluripotency. bioRxiv, 2023-09.

* PD0325901, XAV939, Go6983, Y27632, CHI99021, A83-01, and SB431542 from Axon Medchem

 

Zorzan, I., Betto, R. M., Rossignoli, G., Arboit, M., Drusin, A., Corridori, C., ... & Martello, G. (2023). Chemical conversion of human conventional PSCs to TSCs following transient naive gene activation. EMBO reports, 24(4), e55235.

* PD0325901, XAV939, Go6983, Y27632, CHI99021, A83-01, and SB431542 from Axon Medchem

 

Warin, J., Vedrenne, N., Tam, V., Zhu, M., Yin, D., Lin, X., ... & Camus, A. Integration of in Vitro and in Vivo Models Defines a Molecular Signature Reference for Human Embryonic Notochordal Cells.

 

Ferlazzo, G. M., Gambetta, A. M., Amato, S., Cannizzaro, N., Angiolillo, S., Arboit, M., ... & Martello, G. (2023). Genome-wide screening in pluripotent cells identifies Mtf1 as a suppressor of mutant huntingtin toxicity. Nature Communications, 14(1), 3962.

* SB431542 + CHIR99021 + Y27632 from Axon Medchem

 

Paluschinski, M., Kordes, C., Vucur, M., Buettner, V., Roderburg, C., Xu, H. C., ... & Castoldi, M. (2023). Differential modulation of miR-122 transcription by TGFβ1/BMP6: implications for nonresolving inflammation and hepatocarcinogenesis. Cells, 12(15), 1955.

 

Zorzan, I., Betto, R. M., Rossignoli, G., Arboit, M., Drusin, A., Martini, P., & Martello, G. (2022). Chemical conversion of human conventional Pluripotent Stem Cells to Trophoblast Stem Cells. bioRxiv, 2022-04.

* CHIR99021 + PD0325901 + XAV927 + GO6983 + Y27632 + A8301 = SB431542 + PD173074 from Axon Medchem

 

Pham, T. X. A., Panda, A., Kagawa, H., To, S. K., Ertekin, C., Georgolopoulos, G., ... & Pasque, V. (2022). Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells. Cell stem cell, 29(9), 1346-1365.

* SB431542 + CHIR99021 + PD0325901 from Axon Medchem

 

Rousset, F., Schilardi, G., Sgroi, S., Nacher-Soler, G., Sipione, R., Kleinlogel, S., & Senn, P. (2022). WNT activation and TGFβ-Smad inhibition potentiate stemness of mammalian auditory neuroprogenitors for high-throughput generation of functional auditory neurons in vitro. Cells, 11(15), 2431.

* CHIR99021 + LDN193189 + SB431542 from Axon medchem

 

Rousset, F., Schilardi, G., Sgroi, S., Nacher-Soler, G., Sipione, R., Kleinlogel, S., & Senn, P. (2022). WNT Activation and TGFβ-Smad Inhibition Potentiate Stemness of Mammalian Auditory Neuroprogenitors for High-Throughput Generation of Functional Auditory Neurons In Vitro. Cells 2022, 11, 2431.

* CHIR99021 + LDN193189 + SB431542 from Axon medchem

 

Zijlmans, D. W., Talon, I., Verhelst, S., Bendall, A., Van Nerum, K., Javali, A., ... & Pasque, V. (2022). Integrated multi-omics reveal polycomb repressive complex 2 restricts human trophoblast induction. Nature cell biology, 24(6), 858-871.

*PD0325901 + CHIR99021 + SB431542 from Axon Medchem

 

Viukov, S., Shani, T., Bayerl, J., Sheban, D., Stelzer, Y., Novershtern, N., & Hanna, J. (2022). Human primed and naïve PSCs are both competent in differentiating into bona fide trophoblast stem cells. BioRxiv, 2022-05.

* CHIR99021 + PD0325901 + A83-01, Birb0796 + XAV927 + Y27632 + SB431542 + CGP77675 + GO6983 from Axon Medchem

 

Manstein, F., Ullmann, K., Kropp, C., Halloin, C., Triebert, W., Franke, A., ... & Zweigerdt, R. (2021). High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling. Stem cells translational medicine, 10(7), 1063-1080.

* CHIR99021 + SB431542 from Axon Medchem

 

Tong, Z., Kwak, E., Aguiar, A., Peng, B., Pouton, C. W., Voelcker, N. H., & Haynes, J. M. (2021). Compartmentalized microfluidic chambers enable long-term maintenance and communication between human pluripotent stem cell-derived forebrain and midbrain neurons. Lab on a Chip, 21(20), 4016-4030.

*Y 27632 + SB431542 from Axon Medchem

 

Varderidou-Minasian, S., Verheijen, B. M., Harschnitz, O., Kling, S., Karst, H., Van Der Pol, W. L., ... & Altelaar, M. (2021). Spinal muscular atrophy patient iPSC-derived motor neurons display altered proteomes at early stages of differentiation. ACS omega, 6(51), 35375-35388.

 

Kwak, E. (2021). Evaluating the effect of Amyloid β on inflammatory response and synaptic health of forebrain neurons derived from human embryonic stem cells (Doctoral dissertation, Monash University).

* LDN193189 + SB431542 _ Y27632 from Axon Medchem

 

Paluschinski, M. M. The Functional Analysis and Characterization of the Liver-Specific MicroRNA miR 122 and of its Associated Target Genes (Doctoral dissertation, Dissertation, Düsseldorf, Heinrich-Heine-Universität, 2020).

 

Castel, G., Meistermann, D., Bretin, B., Firmin, J., Blin, J., Loubersac, S., ... & David, L. (2020). Induction of human trophoblast stem cells from somatic cells and pluripotent stem cells. Cell Reports, 33(8).

× Y27632, SB431541, CHIR99021, A83-01, PD0325901, GO6983, IWR-1-endo, Foskolin from Axon Medchem

 

Morsing, M., Kim, J., Villadsen, R., Goldhammer, N., Jafari, A., Kassem, M., ... & Rønnov-Jessen, L. (2020). Fibroblasts direct differentiation of human breast epithelial progenitors. Breast Cancer Research, 22, 1-16.

 

Zorzan, I., Pellegrini, M., Arboit, M., Incarnato, D., Maldotti, M., Forcato, M., ... & Martello, G. (2020). The transcriptional regulator ZNF398 mediates pluripotency and epithelial character downstream of TGF-beta in human PSCs. Nature Communications, 11(1), 2364.

* Y27632, SB431542, LDN193189, A83-01 from Axon Medchem

 

Meijer, M., Rehbach, K., Brunner, J. W., Classen, J. A., Lammertse, H. C., van Linge, L. A., ... & Verhage, M. (2019). A single-cell model for synaptic transmission and plasticity in human iPSC-derived neurons. Cell reports, 27(7), 2199-2211.

* SB431542 + LDN193189 from Axon Medchem

 

Rhee, H. J., Shaib, A. H., Rehbach, K., Lee, C., Seif, P., Thomas, C., ... & Rhee, J. S. (2019). An autaptic culture system for standardized analyses of iPSC-derived human neurons. Cell reports, 27(7), 2212-2228.

* SB431542 + LDN193189 from Axon Medchem

 

Ito, Y., Nakamura, S., Sugimoto, N., Shigemori, T., Kato, Y., Ohno, M., ... & Eto, K. (2018). Turbulence activates platelet biogenesis to enable clinical scale ex vivo production. Cell, 174(3), 636-648.

* CHIR99021, SB431542, IOX2, PRT4165, LY294002 from Axon Medchem

 

Hohwieler, M., Illing, A., Hermann, P. C., Mayer, T., Stockmann, M., Perkhofer, L., ... & Kleger, A. (2017). Human pluripotent stem cell-derived acinar/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling. Gut, 66(3), 473-486.

* CHIR99021 + SB431542 from Axon Medchem

 

Hopkinson, B. M., Klitgaard, M. C., Petersen, O. W., Villadsen, R., Rønnov-Jessen, L., & Kim, J. (2017). Establishment of a normal-derived estrogen receptor-positive cell line comparable to the prevailing human breast cancer subtype. Oncotarget, 8(6), 10580.

* Y27632 + SB431542 from Axon Medchem

 

Rademacher, S., Verheijen, B. M., Hensel, N., Peters, M., Bora, G., Brandes, G., ... & Claus, P. (2017). Metalloprotease-mediated cleavage of PlexinD1 and its sequestration to actin rods in the motoneuron disease spinal muscular atrophy (SMA). Human molecular genetics, 26(20), 3946-3959.

* Y27632 + SB431542 from Axon Medchem

 

Rademacher, S. (2017). Cytoskeletal dysregulation in the motoneuron disease Spinal Muscular Atrophy (SMA) (Doctoral dissertation, Bibliothek der Tierärztlichen Hochschule Hannover).

* Y27632 + SB431542 fro Axon Medchem

 

Alzubi, J., Pallant, C., Mussolino, C., Howe, S. J., Thrasher, A. J., & Cathomen, T. (2017). Targeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model. Scientific Reports, 7(1), 12475.

* CHIR99021 + PD0325901 + SB431542 from Axon medchem

 

Harschnitz, O., van den Berg, L. H., Johansen, L. E., Jansen, M. D., Kling, S., Vieira de Sá, R., ... & van der Pol, W. L. (2016). Autoantibody pathogenicity in a multifocal motor neuropathy induced pluripotent stem cell–derived model. Annals of Neurology, 80(1), 71-88.

*Y27632 and SB431542 from Axon Medchem

 

Degueurce, G., d'Errico, I., Pich, C., Ibberson, M., Schütz, F., Montagner, A., ... & Michalik, L. (2016). Identification of a novel PPARβ/δ/miR‐21‐3p axis in UV‐induced skin inflammation. EMBO molecular medicine, 8(8), 919-936.

 

Sudheer, S., Liu, J., Marks, M., Koch, F., Anurin, A., Scholze, M., ... & Herrmann, B. G. (2016). Different concentrations of FGF ligands, FGF2 or FGF8 determine distinct states of WNT-induced presomitic mesoderm. Stem cells, 34(7), 1790-1800.

*CHIR99021, PD0325901 and SB431542 from Axon Medchem

 

Linnartz‐Gerlach, B., Schuy, C., Shahraz, A., Tenner, A. J., & Neumann, H. (2016). Sialylation of neurites inhibits complement‐mediated macrophage removal in a human macrophage‐neuron Co‐Culture System. Glia, 64(1), 35-47.

*CHIR99021 and SB431542 from Axon Medchem