Potent activator of large-conductance Ca2+-activated potassium channels (BK, KCa1.1, MaxiK; EC50 value 11 µM in a Tl+ assay) with a favorable selectivity profile in a screen of 68 receptors and by functional tests on Nav, Cav, SK, and IK channels. NS19504 potently inhibits urinary bladder spontaneous phasic contractions (SPCs) while having only a modest effect on contractions evoked by electrical field stimulation (EFS) and no effect on high K+-induced contractions. At a concentration of 10 µM, NS19504 was also found to inhbit the σ1 receptor, two transporters of neurotransmiters (DA and Norepinephrine), and soluble epoxide hydrolase (sEH).